Looking to improve your routine PCR?
Bestaq™ DNA Polymerase is the new standard.
With outstanding PCR yield, exceptional fidelity and high processivity, crb’s Bestaq™ DNA Polymerase is a versatile enzyme ideal for all PCR applications. With this superior enzyme, you can consolidate all PCR protocols and reactions into one efficient system.
Benefits crb’s Bestaq™ DNA Polymerase
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Routine amplification in a cost-effective way? crb’s Taq DNA Polymerase is the answer. crb’s Taq DNA polymerase offers consistent results across a wide range of DNA templates with excellent yield and sensitivity. This conventional polymerase is most suitable for PCR applications where less emphasis is required on fidelity. Benefits of crb’s Taq DNA Polymerase - Robust PCR performance with great reproducibility
- High sensitivity
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Extraction-free DNA amplification from whole blood samples with crb’s Bloodirect DNA Polymerase. With this unique enzyme, DNA can be amplified in reactions containing up to 20% (v/v) whole blood sample without a separate DNA purification step. As a result, the use of crb’s Bloodirect DNA Polymerase offers an overall reduction in contamination risk, experimental run time, and cost of genetic testing. Benefits of crb’s Bloodirect DNA Polymerase - Eliminate lengthy DNA purification steps
- Achieve direct amplification from fresh or frozen blood preserved in EDTA, citrate or heparin
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SensTaq HotStart DNA Polymerase is a new type of thermal stable DNA polymerase that is fused with a dsDNA binding subunit This feature allows the DNA polymerase to stay binding on the template for longer, thus improving its sensitivity and processivity. The enzyme is also an antibody-based hotstart DNA polymerase which gives the enzyme a very good specificity. The proprietary antibody blocks polymerase activity at low temperatures. During the initial denaturation step, the antibody dissociates from the polymerase and restores enzyme activity. Therefore SensTaq is an ideal choice for detection assays and limited template situation such as single-cell target amplification. SensTaq HotStart DNA Polymerase has 5’ to 3’ exonuclease activity but lacks 3’ to 5’ proofreading activity, and it is able to amplify products up to 1.5kb in length containing a single base (A) overhang on the 3’ ends. Quantity: 250U -
Streamline your PCR with crb’s 2X PCR MasterMix. crb’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, crb offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. crb’s MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel. Benefits of 2X PCR Taq MasterMix - Robust PCR performance with great reproducibility
- High sensitivity
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Streamline your PCR with crb’s 2X PCR MasterMix. crb’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, crb offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. crb’s MasterMix with dye includes, in addition, an inert blue dye (Bromophenol Blue, with migration equivalent to 500bp DNA fragment) and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel. Benefits of 2X PCR Taq MasterMix with dye • Robust PCR performance with great reproducibility • High sensitivity Quantity: 5.0 ml (200 Rxns) -
The use of TRIzol® in gene expression studies involving routine RNA extraction often poses bio-hazard and health concerns to scientist and researchers.ExCellenCT Lysis Kit is crb’s unique and effective alternative method for RNA template extraction and preparation. RNA templates are extracted and processed directly from cultured cells for one-step or two-steps qRT-PCR in less than 15 minutes, unveiling reverse-transcription-ready RNA/cell lysate from cultured cells without the time-consuming and hazardous-chemicals-involved RNA extraction and purification steps. RNA templates generated can then be directly used as template in either a one-step qRT-PCR or a two-step qRT-PCR system. In addition, samples can be directly processed in culture wells (96 and 384 wells) for easy handling and high throughput, thus minimizing transfer error and potential sample loss. This product is only intended for quick RNA extraction from cultured cells for two-steps RT-PCR or two-steps qRT-PCR gene expression analysis. This product is not suitable for typical RNA/cDNA pool preparation. Quantity: 25 Preps -
The In vitro DNA Amplification Kit now provides end users with a faster and more efficient technique for high yield DNA amplification from minimal starting template With a simple set up and short handling time, this has now become the method of choice for any application requiring high throughput DNA amplification. The hassle-free protocol eliminates the requirement for mini prep DNA isolation, thus significantly reducing the cost and time required to obtain your amplified product from the reaction. Applications of this technology include (but are not limited to) whole genome amplification, DNA library construction, SNP genotyping and in vitro cloning of lethal DNA. Quantity: 100 Reactions -
dNTP Set consists of 100mM aqueous solutions of dATP, dCTP, dGTP and dTTP each supplied in a separate vial Since the nucleotides are provided separately, the dNTP Set offers maximum flexibility in preparation of reaction mixes for different applications. Quantity: 250 µl, 10 mM each
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Choose your priming methods with crb’s cDNA Synthesis Kit crb’s cDNA Synthesis kit provides a comprehensive set of reagents necessary to generate high-quality cDNA and offers the most flexibility in respect to priming methods and reaction optimization. Both random primers and oligo(dT) are included for a choice of general priming strategies and as alternatives to gene-specific primers. Benefits of OneScript® cDNA Synthesis Kit - Maximal flexibility in priming – oligo(dT), random primers or gene-specific primers
- Robust cDNA synthesis from any RNA template
- High reproducibility and excellent yield
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Convenient and time-saving reaction set-up with crb’s cDNA Synthesis SuperMix crb’s cDNA Synthesis SuperMix provides highly efficient and specific conversion of RNA to cDNA, even from low-abundance transcripts. Furthermore, the unique blend of random primers and oligo(dT) primers in this SuperMix ensures optimal representation of all transcript sequences in the cDNA product. Overall, crb’s cDNA Synthesis SuperMix allows you to perform first-strand cDNA synthesis with minimal effort and maximum ease. Benefits of OneScript® cDNA Synthesis SuperMix - Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
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Convenient and time-saving reaction set-up with crb’s OneScript® Two-Step RT-PCR Kit This highly efficient two-step RT-PCR kit consists of two main components: OneScript® cDNA Synthesis SuperMix and TaqPlus 2X PCR MasterMix with dye. crb’s cDNA Synthesis SuperMix provides highly efficient and specific conversion of RNA to cDNA, even from low-abundance transcripts. Furthermore, the unique blend of random primers and oligo(dT) primers in this SuperMix ensures optimal representation of all transcript sequences in the cDNA product. Overall, crb’s cDNA Synthesis SuperMix allows you to perform first-strand cDNA synthesis with minimal effort and maximum ease. crb’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, crb offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. crb’s MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel. Benefits of OneScript® Two-Step RT-PCR Kit - Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield and robust PCR performance
