| Streamline your PCR with crb’s 2X PCR MasterMix. crb’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, crb offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. crb’s MasterMix with dye includes, in addition, an inert blue dye (Bromophenol Blue, with migration equivalent to 500bp DNA fragment) and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel. Benefits of 2X PCR Taq Plus MasterMix • Improved sensitivity and fidelity compared to conventional Taq DNA Polymerase • Robust and consistent performance across a wide range of templates • An economical alternative to Taq DNA Polymerase Quantity: 25.0 ml (1000 Rxns) |
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Streamline your PCR with crb’s 2X PCR MasterMix. crb’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, crb offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. crb’s MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel. Benefits of 2X PCR Taq Plus MasterMix • Improved sensitivity and fidelity compared to conventional Taq DNA Polymerase • Robust and consistent performance across a wide range of templates • An economical alternative to Taq DNA Polymerase Quantity: 5.0 ml (200 Rxns) -
Streamline your PCR with crb’s 2X PCR MasterMix. crb’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, crb offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. crb’s MasterMix with dye includes, in addition, an inert blue dye (Bromophenol Blue, with migration equivalent to 500bp DNA fragment) and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel. Benefits of 2X PCR Taq Plus MasterMix with dye • Improved sensitivity and fidelity compared to conventional Taq DNA Polymerase • Robust and consistent performance across a wide range of templates • An economical alternative to Taq DNA Polymerase Quantity: 25.0 ml (1000 Rxns) -
Streamline your PCR with crb’s 2X PCR MasterMix. crb’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, crb offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. crb’s MasterMix with dye includes, in addition, an inert blue dye (Bromophenol Blue, with migration equivalent to 500bp DNA fragment) and a stabilizer which allow for loading of the PCR product(s) onto an agarose gel. Benefits of 2X PCR Taq Plus MasterMix with dye • Improved sensitivity and fidelity compared to conventional Taq DNA Polymerase • Robust and consistent performance across a wide range of templates • An economical alternative to Taq DNA Polymerase Quantity: 5.0 ml (200 Rxns) -
Streamline your PCR with crb’s 2X PCR MasterMix crb’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, crb offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. crb’s MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel. Benefits of 2X PCR TaqFast MasterMix • High-speed PCR without compromising accuracy • High-processivity to reduce reaction time by 80% Quantity: 5.0 ml (200 Rxns) -
Streamline your PCR with crb’s 2X PCR MasterMix crb’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, crb offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. crb’s 2X PCR TaqFast MasterMix with dye contains a green dye blend which resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel. Benefits of 2X PCR TaqFast MasterMix with dye • High-speed PCR without compromising accuracy • High-processivity to reduce reaction time by 80% Quantity: 5.0 ml (200 Rxns) -
From RNA template to cDNA in just 15 minutes completely hassle-free With crb’s 5X All-In-One RT MasterMix, just add RNA and this optimized system will provide sensitive and reliable cDNA synthesis over a dynamic range of input RNA. The use of this MasterMix eliminates multiple component additions providing exceptional reproducibility and precision, in addition to offering the end-user ultimate convenience. Benefits of 5X All-In-One RT MasterMix • Reduction in handling errors with only 1 liquid transfer step • Streamlined protocol suitable for high-throughput applications • Simple set-up for any RNA template • High reproducibility and excellent yield
Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup. Quantity: 25 x 10 µl reactions -
From RNA template to cDNA in just 15 minutes completely hassle-free With crb’s 5X All-In-One RT MasterMix, just add RNA and this optimized system will provide sensitive and reliable cDNA synthesis over a dynamic range of input RNA. The use of this MasterMix eliminates multiple component additions providing exceptional reproducibility and precision, in addition to offering the end-user ultimate convenience. Benefits of 5X All-In-One RT MasterMix • Reduction in handling errors with only 1 liquid transfer step • Streamlined protocol suitable for high-throughput applications • Simple set-up for any RNA template • High reproducibility and excellent yield
Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup. Quantity: 100 x 10 µl reactions -
From RNA template to cDNA in just 15 minutes completely hassle-free With crb’s 5X All-In-One RT MasterMix, just add RNA and this optimized system will provide sensitive and reliable cDNA synthesis over a dynamic range of input RNA. The use of this MasterMix eliminates multiple component additions providing exceptional reproducibility and precision, in addition to offering the end-user ultimate convenience. Benefits of 5X All-In-One RT MasterMix • Reduction in handling errors with only 1 liquid transfer step • Streamlined protocol suitable for high-throughput applications • Simple set-up for any RNA template • High reproducibility and excellent yield
Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup. Quantity: 200 x 10 µl reactions -
From RNA template to cDNA in just 15 minutes completely hassle-free With crb’s 5X All-In-One RT MasterMix, just add RNA and this optimized system will provide sensitive and reliable cDNA synthesis over a dynamic range of input RNA. The use of this MasterMix eliminates multiple component additions providing exceptional reproducibility and precision, in addition to offering the end-user ultimate convenience. Benefits of 5X All-In-One RT MasterMix - Reduction in handling errors with only 1 liquid transfer step
- Streamlined protocol suitable for high-throughput applications
- Simple set-up for any RNA template
- High reproducibility and excellent yield
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Removal of contaminating genomic DNA from your samples to reverse transcription of RNA in 30 minutes, with 5X All-In-One RT MasterMix (AccuRT Genomic DNA Removal Kit included) crb’s 5X All-in-One RT MasterMix (with AccuRT Genomic DNA Removal Kit) offers a convenient and complete system to facilitate the complete elimination of contaminating genomic DNA in RNA preparations for rapid synthesis of high-quality cDNA for downstream applications. Benefits of 5X All-In-One RT MasterMix (with AccuRT Genomic DNA Removal Kit) • Efficient ds and ssDNA digestion in under 10 minutes • Maximal RNA protection – No heating or organic extraction steps required • Fewer handling errors, with only 1 liquid transfer step for reverse transcription • Streamlined protocol suitable for high-throughput applications • Simple set-up for any RNA template • High reproducibility and excellent cDNA yield
Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup. Quantity: 100 x 20 µl reactions -
Extracting and preparing RNA templates directly from cultured cells to be reverse-transcribed into cDNA, with 5X All-In-One RT MasterMix crb’s ExCellenCT Lysis Kit is an unique and effective alternative method to provide RNA templates that are extracted and processed directly from cultured cells in less than 15 minutes, unveiling reverse-transcription-ready RNA/cell lysate from cultured cells without the time-consuming and hazardous-chemicals-involved RNA extraction and purification steps. RNA templates generated can then be directly used as template to be reverse-transcribed into cDNA using the 5X All-In-One RT MasterMix. The 5X All-In-One RT MasterMix is a ready-to-use formulation of all the reagents necessary for first-strand cDNA synthesis, including crb’s proprietary OneScript® Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, dNTPs, and a finely balanced ratio of Oligo (dT)s and Random Primers. Coupled together, this complete system provides the ultimate convenience in generating high-quality cDNA directly from 10-105 cultured cells, suitable for a wide range of downstream applications. This product is only intended for quick RNA extraction from cultured cells for two-steps RT-PCR or two-steps qRT-PCR gene expression analysis. This product is not suitable for typical RNA/cDNA pool preparation. Quantity: 25 Preps, 100 x 20 µl reactions
