TaqProbe 5X qPCR MasterMix-Multiplex

337$

Ultimate sensitivity in real-time PCR with crb’s TaqProbe 5X qPCR MasterMix

crb’s TaqProbe 5X qPCR MasterMix is designed for high throughput quantitative PCR using TaqMan® probe-based chemistry. Available with the option of ROX or fluorescein as the internal passive reference dye, crb’s TaqProbe 5X qPCR MasterMix offers superb performance in sensitivity and signal-to-noise ratio. The multiplex formulation supports quantitative amplification and detection of up to four targets simultaneously with consistent and reliable results.

Benefits of TaqProbe 5X qPCR MasterMix-Multiplex

  • Antibody hot-start technology
  • Enable streamlined protocol in a simple reaction set-up
  • Allow accurate quantification of a variety of gene targets
  • Reduce pipetting steps to minimize the risk of contamination
  • Compatible with most real-time PCR instruments

Please refer to our qPCR MasterMix Selection Guide for selecting the appropriate qPCR formulation applicable to your particular instrument model.

 

Quantity: 4 x 1.0 ml, for 1000 reactions (20 µl)

 

SKU: MasterMix-5PM Categories: , , , , Tag:

Description

Data Sheet

Literature

 

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Application

 

 • Gene-expression analysis
• Gene knockdown validation
• SNP genotyping assays
• CHiP
• Copy number variation
• Microarray validation
• High throughput applications
• Virus detection and quantification
Picture 1
Protocol

 

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Storage

 

 Stored at -20°C and protect from light.

 
Notes 1. Aliquot reagents to avoid contamination and to avoid repeated freeze-thaw cycles.
2. Primer concentration should not be high, a concentration of 100 nM to 300 nM of each primer usually gives the best results.
3. A very effective way to get tight Ct among replicates is to reduce pipetting error, this can be achieved by: preparing amplicon specific pre-mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range.
4. For optimal results, it is recommended that the primers are 18-22 nucleotides in length with a Tm of 58°C-60°C and the size of target is about 100-250 bp.This product is ISO-13485 certified.

 

 

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