BrightGreen Express 2X qPCR MasterMix-iCycler

125$

Guaranteed high-performance real-time PCR using crb’s BrightGreen Express 2X qPCR MasterMix

crb’s BrightGreen Express 2X qPCR MasterMix provides all ingredients necessary for extremely rapid quantitative PCR in a premixed and optimized format. The use of crb’s TaqFast DNA Polymerase in BrightGreen Express 2X qPCR MasterMix shortens the reaction time to about 1/5 of standard qPCR time. Available with the option of ROX or fluorescein as the internal passive reference dye, crb’s BrightGreen Express 2X qPCR MasterMix offers unparalleled performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers.

Benefits of BrightGreen Express 2X qPCR MasterMix-iCycler

• Extremely rapid activation – only 30 seconds!
• Ultra-fast qPCR thermal cycling – 10 second annealing/elongation time
• Streamlined protocol with a simple reaction set-up
• Ultra-fast quantification of a variety of gene targets
• Reduces pipetting steps to minimize the risk of contamination
• Compatible with most real-time PCR instruments.

Please refer to our BrightGreen Express qPCR MasterMix Selection Guide (found in the Documents section below) for selecting the appropriate qPCR formulation applicable to your particular instrument model.

 

Quantity: 4 x 1.25 ml, for 500 reactions (20 µl)

 

 

 

Description

Data Sheet

Application  

• Gene-expression analysis
• Gene knockdown validation
• Microarray validation
• High throughput applications
• Virus detection and quantification

 

 Protocol  

 

Click To Open

 

Picture 1
Picture 2
 Concentration  

 

2X

 

Storage  

 

Stored at -20°C and protect from light.

 

Notes  

1. Aliquot reagents to avoid contamination and to avoid repeated freeze-thaw cycles.
2. Primer concentration should not be high, a concentration of 100 nM to 300 nM of each primer usually gives the best results.
3. A very effective way to get tight Ct among replicates is to reduce pipetting error, this can be achieved by: preparing amplicon specific pre-mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range.
4. For optimal results, it is recommended that the primers are 18-22 nucleotides in length with a Tm of 58°C-60°C and the size of target is about 100-250 bp.This product is distributed for laboratory research only.
Caution: Not for diagnostic use.