| The best companion for tissue PCR crb’s Tissue Ex-Amp PCR Kit offers the ultimate convenience for animal sample PCR. The hassle-free gDNA extraction process eliminates the needs of conventional organic extraction, column DNA purification, or alcohol precipitation. Unlike other extraction buffers on the market which require an extra neutralization step during the extraction process, crb’s advanced proprietary extraction buffer only takes 15 easy minutes to “incubate & boil” a small amount of animal tissue, cell, or fluid sample to obtain PCR-ready template without re-opening the extraction tube, thus minimizing the risk of contamination and human error. The kit comes with crb’s sophisticated Kodaq 2X PCR MasterMix with dye; the mastermix is not only for un-beatable robustness and extreme fidelity, but also for a very streamlined and efficient PCR setup. No more under/over-run agarose gel electrophoresis: Kodaq 2X PCR MasterMix with dye contains a green dye blend which resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel. Quantity: 100 preps, 120 x 50ul reactions |
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A complete RNA quantification by revealing all the different RNAs in a sample crb’s Total-Transcriptome cDNA Synthesis kit provides all reagents necessary to generate high quality cDNA from all different kind of RNA in a sample such as mRNA, miRNA, and lncRNA. This is made possible by 3’ poly(A)-tailing of RNAs via the use of poly(A) polymerase. Oligo d(T) adaptor and random primers then anneal onto the RNA template and facilitate the reverse transcription. OneScript® Plus Reverse Transcriptase is a novel recombinant reverse transcriptase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. The OneScript® Plus Reverse Transcriptase is engineered to perform under high temperatures (50°C - 55°C); facilitating the elimination of secondary structures associated with GC-rich RNA templates. The recombinant RNaseOFF Ribonuclease Inhibitor effectively protects RNA templates from degradation. Also the universal 3’ reverse primer included in the kit can be coupled with any specific forward primer in downstream qPCR/PCR amplification. Benefits of Total-Transcriptome cDNA Synthesis Kit - Revealing all the RNAs in a human or mouse RNA sample
- A complete, comprehensive cDNA synthesis kit
- Including oligo dT adaptor and Universal reverse primer for high efficiency
- Including positive control assays for mRNA, miRNA, and lncRNA studies
- Robust cDNA synthesis from any RNA template
- High reproducibility and excellent yield
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A complete RNA quantification by revealing all the different RNAs in a sample crb’s Total-Transcriptome cDNA Synthesis kit provides all reagents necessary to generate high quality cDNA from all different kind of RNA in a sample such as mRNA, miRNA, and lncRNA. This is made possible by 3’ poly(A)-tailing of RNAs via the use of poly(A) polymerase. Oligo d(T) adaptor and random primers then anneal onto the RNA template and facilitate the reverse transcription. OneScript® Plus Reverse Transcriptase is a novel recombinant reverse transcriptase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. The OneScript® Plus Reverse Transcriptase is engineered to perform under high temperatures (50°C - 55°C); facilitating the elimination of secondary structures associated with GC-rich RNA templates. The recombinant RNaseOFF Ribonuclease Inhibitor effectively protects RNA templates from degradation. Also the universal 3’ reverse primer included in the kit can be coupled with any specific forward primer in downstream qPCR/PCR amplification. Benefits of Total-Transcriptome cDNA Synthesis Kit - Revealing all the RNAs in a human or mouse RNA sample
- A complete, comprehensive cDNA synthesis kit
- Including oligo dT adaptor and Universal reverse primer for high efficiency
- Including positive control assays for mRNA, miRNA, and lncRNA studies
- Robust cDNA synthesis from any RNA template
- High reproducibility and excellent yield
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crb's ultRNA Column Purification Kit is a fast and efficient spin column method to purify total RNA from cells or tissues. Cells and tissues can be lysed and homogenized to release RNA, which then binds to the silica membrane filter in the RNA binding column. Purified total RNA eluted from the column will be of suitable quality for various downstream molecular biology applications such as RT-PCR, qRT-PCR, Northern blotting and more RNA-based analyses. The ultRNA Column Purification Kit can also be used to clean up RNA extracted by different methods or RNA samples from a variety of reactions. The ultRNA Plus Column Purification kit includes a gDNA filter designed to shear genomic DNA completely, minimize sample viscosity and DNA contamination. This kit is recommended for use with tissues and cell samples (>106 cells). Quantity: 50 Preparations
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crb's ultRNA Column Purification Kit is a fast and efficient spin column method to purify total RNA from cells or tissues. Cells and tissues can be lysed and homogenized to release RNA, which then binds to the silica membrane filter in the RNA binding column. Purified total RNA eluted from the column will be of suitable quality for various downstream molecular biology applications such as RT-PCR, qRT-PCR, Northern blotting and more RNA-based analyses. The ultRNA Column Purification Kit can also be used to clean up RNA extracted by different methods or RNA samples from a variety of reactions. The ultRNA Plus Column Purification kit includes a gDNA filter designed to shear genomic DNA completely, minimize sample viscosity and DNA contamination. This kit is recommended for use with tissues and cell samples (>106 cells). Quantity: 50 Preparations
