Extraction-free DNA amplification from whole blood samples with crb’s Bloodirect DNA Polymerase.
With this unique enzyme, DNA can be amplified in reactions containing up to 20% (v/v) whole blood sample without a separate DNA purification step. As a result, the use of crb’s Bloodirect DNA Polymerase offers an overall reduction in contamination risk, experimental run time, and cost of genetic testing.
Benefits of crb’s Bloodirect DNA Polymerase
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Synthesize cDNA from only 0.1pg of RNA with crb’s OneScript Reverse Transcriptase Using strategic modifications, crb’s OneScript Reverse Transcriptase lacks the intrinsic RNase H acitivity thereby drastically increases the yield and achievable synthesized cDNA length (up to 9kb). In addition, OneScript contains a fidelity-enhancing subunit which ensures superior accuracy in reverse transcription reaction(s). Quantity: 25 x 20 µl reactions -
Cost-effective quick PCR with improved fidelity? With crb’s Taq Plus DNA Polymerase. crb’s Taq Plus DNA polymerase employs an optimized two-polymerase blend that provides high product yield, sensitivity and fidelity. Taq Plus DNA Polymerase can be used in a variety of general PCR experiments, providing improved efficiency and performance at a reduced cost. Benefits of crb’s Taq Plus DNA Polymerase - Improved sensitivity and fidelity compared to conventional Taq DNA Polymerase
- Robust and consistent performance across a wide range of templates
- An economical alternative to Taq DNA Polymerase
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Short on time? crb’s TaqFast DNA Polymerase to the rescue. With extension rate as fast as 10 sec/kb (compared to 60 sec/kb with regular Taq), crb’s TaqFast DNA polymerase offers the ultimate speed to dramatically reduce total reaction time. In addition to improved processivity, TaqFast DNA polymerase also possesses moderate 3’-5’ proofreading activity, making this enzyme well suited for high-throughput PCR. Benefits of crb's TaqFast DNA Polymerase - High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by 80%
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From RNA template to cDNA in just 15 minutes completely hassle-free With crb’s 5X All-In-One RT MasterMix, just add RNA and this optimized system will provide sensitive and reliable cDNA synthesis over a dynamic range of input RNA. The use of this MasterMix eliminates multiple component additions providing exceptional reproducibility and precision, in addition to offering the end-user ultimate convenience. Benefits of 5X All-In-One RT MasterMix • Reduction in handling errors with only 1 liquid transfer step • Streamlined protocol suitable for high-throughput applications • Simple set-up for any RNA template • High reproducibility and excellent yield
Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup. Quantity: 25 x 10 µl reactions -
Struggling to amplify long targets? crb’s Long-Range DNA Polymerase will end your fight. crb’s Long-Range DNA polymerase is capable of amplifying templates up to 20kb with minimal optimization. Coupled with superb performance, this enzyme’s enhanced extension rate drastically reduces the overall reaction time. crb’s Long-Range DNA polymerase is the ideal choice for generating long PCR products with extreme sensitivity and processivity. Benefits of crb’s Long-Range DNA Polymerase - High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by up to 70%
- Efficient amplification of DNA templates up to 20kb
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Problems with non-specific amplification and primer-dimer formation? Try crb’s HotStart DNA Polymerase. HotStart DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures. During the initial denaturation step at 94°C, the antibody dissociates from Taq DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formations that would otherwise compete for reagent availability. Thus, crb’s HotStart DNA Polymerase offers improved yield of desired PCR products. Benefits of crb’s HotStart DNA Polymerase - The highest specificity with minimal background
- Superior performance
- Improved yield of desired product
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Synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content) with crb’s OneScript Plus Reverse Transcriptase Engineered to perform under high temperatures (45°C - 55°C), crb’s OneScript Plus Reverse Transcriptase can synthesize full-length cDNA libraries from RNA templates up to 15kb in length. In addition, OneScript Plus Reverse Transcriptase has outstanding proofreading ability due to the presence of a fidelity-enhancing subunit, thus making this RTase an excellent choice for whole genome sequencing. Quantity: 25 x 20 µl reactions -
crb’s AccuRT Genomic DNA Removal Kit offers a quick and easy method to eliminate genomic DNA (gDNA) contamination in RNA samples The presence of genomic DNA in RNA preparations is often a significant problem for downstream applications. This contamination often leads to false-positive signals and misrepresentation of gene expression levels. This kit employs a brief reaction of your RNA sample with the enclosed Reaction Mix that will effectively remove gDNA without loss or degradation of RNA. The treated gDNA-free RNA is ready for any downstream application such as RT-PCR and qRT-PCR using crb’s cDNA synthesis or RT-PCR reagents Quantity: 200 Reactions -
crb's Kodaq DNA Polymerase is the solution for a robust PCR system with high fidelity and yield. crb’s Kodaq DNA Polymerase is a novel DNA polymerase with strategically engineered mutations resulting in a robust, high fidelity polymerase. Kodaq DNA polymerase has exceptional 3’ to 5’ exonuclease activity that endows it with superior accuracy over competitor polymerases. This novel enzyme has intrinsically high processivity and is engineered to have an improved binding affinity for DNA resulting in highly successful PCR. The Kodaq DNA Polymerase advanced and sophisticated buffer system not only tolerates high AT and GC content, but also many PCR inhibitors commonly found in a typical DNA sample. Benefits of crb's Kodaq DNA Polymerase - High fidelity PCR
- Robust PCR performance, resistant to most PCR inhibitors commonly found in samples (including plant samples)
- PCR success with A/T and G/C rich templates
- Highly processive, for high yield amplification
