HotStart DNA Polymerase

172$

 

Problems with non-specific amplification and primer-dimer formation?

Try crb’s HotStart DNA Polymerase. 

HotStart DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures. During the initial denaturation step at 94°C, the antibody dissociates from Taq DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formations that would otherwise compete for reagent availability. Thus, crb’s HotStart DNA Polymerase offers improved yield of desired PCR products.

Benefits of crb’s HotStart DNA Polymerase

  • The highest specificity with minimal background
  • Superior performance
  • Improved yield of desired product

 

Quantity: 1,000 U (200 µl)

 

Description

Data Sheet

 

Literature

 

Click To Open

 

Application

 

• Assays with prolonged reaction setup or liquid handling
• Multiplex PCR
• Specific amplification of difficult templates (i.e. G-C rich)
• Low copy PCR assays
• TA Cloning
Form

 

Enzyme supplied with 10X buffer and MgSO4

 

Picture 1

 

 

 Picture 2

 

 

Protocol

 

Click To Open

 

Concentration

 

5U/µl

 

Storage

 

Store all components at -20°C.

 

Notes

 

This product is ISO-13485 certified.