| Streamline your PCR with crb’s 2X PCR MasterMix. crb’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, crb offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. crb’s MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel.
The use of Taq Plus DNA Polymerase in this MasterMix offers: |
2X PCR Taq Plus MasterMix (Copy)
90$
Description
Data Sheet
| Literature |  |
| Application | • Routine PCR amplification of DNA templates up to 6 kb • Suitable for a wide range of PCR assays • TA cloning |
| Form | 5 x 1.0 ml vials |
| Picture 1 |  |
| Protocol | |
| Concentration | 2X |
| Storage | Store at -20°C. |
| Notes | This product is ISO-13485 certified. |
Product Documents
Supporting Protocol
- PCR RT-PCR qPCR Application Handbook
MSDS
- 2X PCR Taq Plus MasterMix
Other
- PCR Product Brochure and Performance Data Version 1.4
Product References
- Chung, G et al. “CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans.” DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.
- Bhagwat, B et al. “An in vivo transient expression system can be applied for rapid and effective selection of artificial microRNA constructs for plant stable genetic transformation.” J Genet Genomics 40 (5):261-270 (2013). DOI: 10.1016/j.jgg.2013.03.012. PubMed: 23706301. Application: PCR.
- Jones, MR et al. “The atm-1 gene is required for genome stability in Caenorhabditis elegans.” Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR.
- Chung, G et al. “CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans.” DNA Repair (Amst.) 10 (11):1174-1182 (2011). DI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.
- Chaiyakul, M et al. “Cytotoxicity of ORF3 proteins from a nonpathogenic and a pathogenic porcine circovirus.” J. Virol. 84:11440-7 (2010). PubMed: 20810737.
- Lee, G et al. “Inhibition of in vitro tumor cell growth by RP215 monoclonal antibody and antibodies raised against its anti-idiotype antibodies.” Cancer Immunol. Immunother. 59 (9):1347-1356 (2010). DOI: 10.1007/s00262-010-0864-7. PubMed: 20473495. Application: PCR.
- Chaiyakul, M et al. “Cytotoxicity of ORF3 proteins from a nonpathogenic and a pathogenic porcine circovirus.” J. Virol. 84 (21):11440-11447 (2010). DOI: 10.1128/JVI.01030-10. PubMed: 20810737
- . Application: PCR.
- Lee, G et al. “Inhibition of in vitro tumor cell growth by RP215 monoclonal antibody and antibodies raised against its anti-idiotype antibodies.” Cancer Immunol. Immunother. 59 (9):1347-1356 (2010). DOI: 10.1007/s00262-010-0864-7. PubMed: 20473495. Application: PCR.
- Ahn, CH et al. “Bacterial biofilm-community selection during autohydrogenotrophic reduction of nitrate and perchlorate in ion-exchange brine.” Appl. Microbiol. Biotechnol. 81 (6):1169-1177 (2009). DOI: 10.1007/s00253-008-1797-3. PubMed: 19066883. Application: PCR.
- Lee, G et al. “Cancer cell expressions of immunoglobulin heavy chains with unique carbohydrate-associated biomarker.” Cancer Biomark 5 (4):177-188 (2009). DOI: 10.3233/CBM-2009-0102. Pubed: 19729827. Application: PCR.
FAQs
- What is the Mg2+ concentration in the buffer?
- How can I optimize the reaction for multiplexing?
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What is the Mg2+ concentration in the buffer?
Final concentration Mg2+ in reaction is 1.5mM.
How can I optimize the reaction for multiplexing?
The polymerase and Mg amounts very often need to be optimized in multiplexing and this will need to be evaluated per case by the end user. Primer concentrations for each amplicon in multiplexing would also need to be optimized. The general rule would be lowering the primer concentrations of the strongly amplified targets and increasing the primer concentrations of the weakly amplified targets.
