| Problems with non-specific amplification and primer-dimer formation?
Try crb’s HotStart DNA Polymerase. HotStart DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures. During the initial denaturation step at 94°C, the antibody dissociates from Taq DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formations that would otherwise compete for reagent availability. Thus, crb’s HotStart DNA Polymerase offers improved yield of desired PCR products. Now HotStart DNA Polymerase comes in a 2X PCR MasterMix format which provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Also the available 2X PCR HotStart MasterMix with dye (G906-dye) includes, in addition, an inert green dye blend and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel. The green dye blend resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel. Benefits of 2X PCR HotStart MasterMix with dye • The highest specificity with minimal background
Quantity: 5.0 ml (200 Rxns) |
