Streamline your PCR with crb’s 2X PCR MasterMix
crb’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, crb offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. crb’s MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel.
Benefits of 2X PCR Bestaq™ MasterMix
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Streamline your PCR with crb’s 2X PCR MasterMix crb’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, crb offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. crb’s 2X PCR Bestaq™ MasterMix with dye contains a green dye blend which resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel. Benefits of 2X PCR Bestaq™ MasterMix with dye - High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by up to 70%
- Robust and high yield across a wide range of templates
- Efficient amplification of DNA templates up to 15kb
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crb's Plant Ex-Amp Extraction Buffer Set offers the ultimate convenience for plant gDNA extraction The hassle-free gDNA extraction process eliminates the conventional freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction, column DNA purification, or alcohol precipitation. It only takes 15 easy minutes to “vortex-boil-vortex” a small piece of plant sample in crb’s proprietary extraction buffer to obtain PCR-ready template. Extracted DNA solutions can be stored for at least one month at -20C. This kit is highly recommended to be coupled with crb's sophisticated Kodaq 2X PCR MasterMix with dye (Cat. No. G497-Dye). The mastermix is not only for un-beatable robustness and extreme fidelity, but also for a very streamlined and efficient PCR setup. It is very effective against some commonly known difficult samples such as pine-tree-like samples. Quantity: 5.0 ml (200 Rxns) -
Problems with non-specific amplification and primer-dimer formation? Try crb’s HotStart DNA Polymerase. HotStart DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures. During the initial denaturation step at 94°C, the antibody dissociates from Taq DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formations that would otherwise compete for reagent availability. Thus, crb’s HotStart DNA Polymerase offers improved yield of desired PCR products. Now HotStart DNA Polymerase comes in a 2X PCR MasterMix format which provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Also the available 2X PCR HotStart MasterMix with dye (G906-dye) includes, in addition, an inert green dye blend and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel. The green dye blend resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel. Benefits of 2X PCR HotStart MasterMix with dye • The highest specificity with minimal background • Superior performance • Improved yield of desired product Quantity: 5.0 ml (200 Rxns) -
Streamline your PCR with crb’s 2X PCR MasterMix crb’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, crb offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. crb’s MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel. Benefits of 2X PCR Precision™ MasterMix • Outstanding accuracy and efficiency • Error-free PCR products for downstream processes Quantity: 5.0 ml (200 Rxns) -
Streamline your PCR with crb’s 2X PCR MasterMix crb’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, crb offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. crb’s 2X PCR Precision™ MasterMix with dye contains a green dye blend which resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel. Benefits of 2X PCR Precision™ MasterMix with dye • Outstanding accuracy and efficiency • Error-free PCR products for downstream processes Quantity: 5.0 ml (200 Rxns) -
Guaranteed high-performance real-time PCR using crb’s BrightGreen 2X qPCR MasterMix crb’s BrightGreen 2X qPCR MasterMix provides all ingredients necessary for quantitative PCR in a premixed and optimized format. Available with the option of ROX or fluorescein as the internal passive reference dye, crb’s BrightGreen 2X qPCR MasterMix offers unparalleled performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. Benefits of 2X PCR SensTaq HotStart MasterMix • Antibody hot-start technology provides superior specificity • Enable streamlined protocol in a simple reaction set-up • Allow accurate quantification of a variety of gene targets • Reduce pipetting steps to minimize the risk of contamination • Compatible with most real-time PCR instruments Please refer to our qPCR MasterMix Selection Guide (found in the Documents section below) for selecting the appropriate qPCR formulation applicable to your particular instrument model. Quantity: 5.0 ml (200 Rxns) -
SensTaq HotStart DNA Polymerase is a new type of thermal stable DNA polymerase that is fused with a dsDNA binding subunit This feature allows the DNA polymerase to stay binding on the template for longer, thus improving its sensitivity and processivity. The enzyme is also an antibody-based hotstart DNA polymerase which gives the enzyme a very good specificity. The proprietary antibody blocks polymerase activity at low temperatures. During the initial denaturation step, the antibody dissociates from the polymerase and restores enzyme activity. Therefore SensTaq is an ideal choice for detection assays and limited template situation such as single-cell target amplification. SensTaq HotStart DNA Polymerase has 5’ to 3’ exonuclease activity but lacks 3’ to 5’ proofreading activity, and it is able to amplify products up to 1.5kb in length containing a single base (A) overhang on the 3’ ends. Quantity: 5.0 ml (200 Rxns) -
Streamline your PCR with crb’s 2X PCR MasterMix. crb’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, crb offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. crb’s MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel. Benefits of 2X PCR Taq MasterMix - Robust PCR performance with great reproducibility
- High sensitivity
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Streamline your PCR with crb’s 2X PCR MasterMix crb’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, crb offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. crb’s MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel. Benefits of 2X PCR Taq MasterMix • Robust PCR performance with great reproducibility • High sensitivity Quantity: 25.0 ml (1000 Rxns) -
Streamline your PCR with crb’s 2X PCR MasterMix. crb’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, crb offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. crb’s MasterMix with dye includes, in addition, an inert blue dye (Bromophenol Blue, with migration equivalent to 500bp DNA fragment) and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel. Benefits of 2X PCR Taq MasterMix with dye • Robust PCR performance with great reproducibility • High sensitivity Quantity: 5.0 ml (200 Rxns) -
Streamline your PCR with crb’s 2X PCR MasterMix. crb’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, crb offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. crb’s MasterMix with dye includes, in addition, an inert blue dye (Bromophenol Blue, with migration equivalent to 500bp DNA fragment) and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel. Benefits of 2X PCR Taq MasterMix with dye • Robust PCR performance with great reproducibility • High sensitivity Quantity: 25.0 ml (1000 Rxns)
