Most next-generation sequencing (NGS) library construction workflows include one or more PCR amplification steps to (i) add functional elements (e.g., sample indices, molecular barcodes or flow cell oligo binding sites), (ii) enrich for sequencing-competent DNA fragments and/or (iii) generate a sufficient amount of library material for downstream processing. PCR is also applied for other purposes in NGS workflows. Whenever PCR is performed, care must be taken to select an appropriate enzyme and protocol parameters, to ensure high efficiency, minimize amplification bias and limit the introduction of sequence artifacts.

Coderegenesis Sequencing Solutions offers a family of crb Kits containing crb DNA Polymerase, engineered using directed evolution technology. The crb enzyme contributes to higher overall sequencing quality and economy by offering high efficiency, low bias amplification of DNA and cDNA for both NGS and PCR applications. This translates to more uniform coverage of difficult regions and lower duplication rates allowing researchers to unlock the potential of their samples.

• Higher and more uniform coverage with lower dropout of difficult regions
• Higher yields, low duplication rates and fewer wasted sequencing reads
• Higher success rates with different sample types/applications
• Convenience with the consistency of one core enzyme
• Trusted as shown through over years in the industry as well as thousands of peer-reviewed publications